원문정보
초록
영어
Translation, process that synthesizes the protein, is related with the cell growth because proteins which synthesized through translation play the major role in the biological system. The modulation of translation elongation rate could affect protein synthesis rate and change the growth rate. There are two factors, EF-G and EF-Tu, involved in the translation elongation step. EF-Tu assists ribosome in recruiting suitable tRNA for mRNA codon sequence. EF-G helps
translocation of ribosome on mRNA. EF-G is a key factor in translation elongation because it determines how fast translocation of ribosome occurs. To modulate the growth rate, a genetic circuit was constructed and applied to on/off switch. fusA gene coding EF-G is regulated by lambda promoter which is repressed by cI repressor. cI repressor is located in the downstream of lac promoter which is activated by induction of IPTG. The regulation system of fusA by cI repressor was validated by measuring the growth rate of BL21 (DE3) deleted chromosomal fusA after IPGT induction. As IPTG concentration was increased, growth rate of BL21 (DE3) was decreased and it is shown that this genetic circuit could control the growth of cell.