earticle

영어

Production of 3-Hydroxypropionic acid (3-HP), often limited by the capability of the enzyme, aldehyde dehydrogenase (ALDH), which converts an intermediate, 3-hydroxypropionaldehyde (3-HPA) to 3-HP. Here we report a new ALDH, PuuC, from K. pneumoniae. The purified PuuC exhibited specificity towards 3-HPA and NAD+ was the preferred coenzyme. The optimum pH and temperature for PuuC activity were pH 8.0 and 45°C, respectively. The Km of 3-HPA and NAD+ were 0.48 and 0.09 mM, respectively. Under aerobic fermentation of glycerol by the recombinant E.coli BL21 carrying the genes (dhaB, gdrAB and puuC) in shake flask produced 52.77 mmol l-1 3-HP with the maximum yield of 0.58 mol mol-1 of glycerol at 24 h.