원문정보
초록
영어
A neutralizing epitope fragment of ApxIIA toxin (ApxIIA#5) of the Korean Actinobacillus pleuropneumoniae serotype 2 strain was expressed and immobilized on the cell surface of S. cerevisiae for efficient vaccine development. Expression of the ApxIIA#5 was confirmed by Western blot analysis using cell wall proteins, and the surface display of the ApxIIA#5 was further visualized under confocal microscopy. Quantitative ELISA revealed that the recombinant ApxIIA#5 directed to the cell surface consisted of approximately 16% cell wall proteins, which is
estimated to be 35 mg of the ApxIIA#5 protein per liter of cultured cells. Immunoassay revealed that the antigen-specific antibodies against ApxIIA#5 were observed in sera of mice that were fed the recombinant ApxIIA#5-expressing yeast, but neither in mice fed the wild-type nor the vector-only transformant. Moreover, mice fed the recombinant epitopeexpressing yeast were protected from an injection of a lethal dose of A. pleuropneumoniae.