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포스터 3분 Speech - 좌장 : 강택진(동국대), 정기준(KAIST)

Optimization of medium composition for butanol production from glycerol by Clostridium pasteurianum DSM525

원문정보

Chuloo MOON, Bohye KIM, Kyung Duk KIM, Byoung-In SANG, Seung Wook KIM, Youngsoon UM

한국생물공학회 한국생물공학회 학술대회 2010 춘계학술대회 및 국제심포지움 2010.04 pp.127-127
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

The medium composition for butanol production by Clostridium pasteurianum DSM525 was optimized using response surface methodology (RSM). A fractional factorial design (FFD) for a total six variables (phosphate buffer, MnSO4·H2O, MgSO4·7H2O, FeSO4·7H2O, yeast extract, and (NH4)2SO4) was applied to identify which variables have significant effect on butanol production. After FFD experiment, FeSO4·7H2O, yeast extract, and (NH4)2SO4 were identified to be the most significant factors. Steepest ascent experiments were applied to estimate the optimal region of the three factors and the central composite experimental design (CCD) was adopted to determine their optimized values. The composition of optimal medium for butanol production
by C. pasteurianum DSM525 was 0.5 g of K2HPO4-KH2PO4, 0.2 g of MnSO4·H2O, 0 g of MgSO4·7H2O, 0.03 g of FeSO4·7H2O, 8~10 g of (NH4)2SO4, 2.7 g of yeast extract per 1 L distilled water. After incubating 48 hours in the optimal composition medium, the final butanol oncentration
was 5.8 g/L which was higher than butanol production (4.5 g/L) with the modified P2 medium which is known for butanol production medium.

키워드

저자정보

  • Chuloo MOON Clean energy center, Korea Institute of Science and Technology, Seoul, Korea, Department of Chemical and biological Engineering, Korea University, Seoul, Korea.
  • Bohye KIM Clean energy center, Korea Institute of Science and Technology, Seoul, Korea, Department of Chemical and biological Engineering, Korea University, Seoul, Korea.
  • Kyung Duk KIM Clean energy center, Korea Institute of Science and Technology, Seoul, Korea.
  • Byoung-In SANG Clean energy center, Korea Institute of Science and Technology, Seoul, Korea.
  • Seung Wook KIM Department of Chemical and biological Engineering, Korea University, Seoul, Korea.
  • Youngsoon UM Clean energy center, Korea Institute of Science and Technology, Seoul, Korea.

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자료제공 : 네이버학술정보

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