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포스터 3분 Speech - 좌장 : 강택진(동국대), 정기준(KAIST)

Transcription factor (MITF) and DNA (E-box) Binding analysis for Pigment Control in Skin

원문정보

Man-Ki SON, Hyang-Bok LEE, Dung HOANG NGUYEN, SunA YOON, Jimin UM, Eun-Ki KIM

한국생물공학회 한국생물공학회 학술대회 2010 춘계학술대회 및 국제심포지움 2010.04 pp.126-126
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

MITF (microphthalmia-associated transcription factor), a bHLH (basic helix-loop-helix) transcription factor with a leucine zipper, is a key regulatory protein for the pigmentation process in melanocytes. Binding of MITF to E-box caused transcription of several pigmenting genes
including the tyrosinase gene. The purpose of this study is to analyze the interaction of MITF and its DNA binding site (E-box, CATGTG) by surface plasmon resonance. Label free DNA-protein interaction assay, SPR (surface plasmon resonance), coated by EDC (Ethyl-3-(3-
dimethylaminopropyl)carbodiimide) / NHS (N-Hydroxysuccinimide) were used with amine coupling methods that permitted efficient immobilization of DNA on the gold surface. It is expected that SPR can be a useful method to study of MITF and E-box interactions.

키워드

저자정보

  • Man-Ki SON Department of Biological Engineering, Inha University, Incheon, Korea.
  • Hyang-Bok LEE Department of Biological Engineering, Inha University, Incheon, Korea.
  • Dung HOANG NGUYEN Department of Biological Engineering, Inha University, Incheon, Korea.
  • SunA YOON Department of Biological Engineering, Inha University, Incheon, Korea.
  • Jimin UM Department of Biological Engineering, Inha University, Incheon, Korea.
  • Eun-Ki KIM Department of Biological Engineering, Inha University, Incheon, Korea.

참고문헌

자료제공 : 네이버학술정보

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