earticle

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The purpose of this research is to produce more amount of fatty acids by improving fatty acid biosynthesis mechanism of Escherichia coli. β-Ketoacyl acyl carrier protein synthase III (fabH) is the enzymes that catalyze the first step in the synthesis of fatty acids with acetyl-CoA
carboxylase (accABC) and malonyl-CoA: acyl carrier protein (fabD) acyltransferase in Escherichia coli MG1655. The fabH, accABC and fabD genes were cloned and characterized. The enzyme of fabH gene leads to increase short-chain-length fatty acids and strong preference for acetyl-CoA, and produces only straight chain fatty acids (SCFAs). β-Ketoacyl acyl carrier protein synthase III (fabH) is also thought to play a role in determining fatty acids type and compositions. The fabH, accABC and fabD genes were identified as homologous gene of E. coli
MG1655 through a metabolic pathway. The recombinant E. coli MG1655 containing the fabH or accABC::fabD or accABC::fabD::fabH geneinserted expression vector (pTrc99A) were constructed. To analyze the effects of gene overexperssion, analysis of fatty acid was performed.