원문정보
초록
영어
To enhance clavulanic acid (CA) production, four structural CA biosynthesis genes ceas2, bls2, cas2 and pah2 carboxyethylarginine synthase, β-lactam synthetase, clavaminate synthase and proclavaminate amidinohydrolase respectively were amplified from Streptomyces clavuligerus NRRL3585 genomic DNA and cloned first in pGEM7 subcloning vector and then integration vector pSET152 and expression vector pIBR25 containing ermE* strong promoter and generated pHN18
and pHN19 respectively. The recombinant plasmids were introduced into S. clavuligerus NRRL3585 by PEG mediated protoplast transformation method. Both recombinant strains produced more CA than the S. clavuligerus parental strain. This study showed that CA production was significantly enhanced by integration and overexpression of potential
biosynthetic genes.