earticle

영어

The atopic dermatitis (AD) is a chronic inflammatory skin illness, with remissions and exacerbations, itch. The important role of mast cells in immediate-type allergic reactions is well recognized. Mast cell activation brings about the process of degranulation that results in the fusion of the cytoplasmic granule membranes with the plasma membrane. A wild rice species, Zizania latifolia is one of the most common emergent species in East Asia and has been used heart disease, diabetes mellitus, skin disease for a long time. In this study, we investigated the effects of extract from Z. latifolia aerial parts on β-hexosaminidase release from mast cell activated by DNPBSA. Degranulation was determined by measuring the release of a granule
marker, β-hexosaminidase. Rat basophilic leukemia(RBL-2H3) cells, a tumor analog of mast cells, display properties of mucosal-type mast cells. The 2H3 cells contain several hundred thousand IgE receptors on the m embrane surface, and after sensitization with mouse monoclonal IgE, the cells respond to antigen and release histamine. Z. latifolia extract significantly inhibited β-hexosaminidase release from mast cell. Inhibition of β-hexosaminidase
release were 0.32, 0.23, 0.10, 0.07, and 0.08 at the treated extract concentration of 10, 25, 50, 100, and 250 ㎍/ml, respectively compared to positive control (0.02 in O.D. 405). This result may provide useful information for further discovering pharmacologically active compounds for
treatment of atopic dermatitis.