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영어
Islet Transplantation is an ideal method for the treatment of type I diabetes.[1] However, there are many problems, which must be solved before entering into clinical trials. Usually, the recipient requires the islets derived from 3-4 donors in order to achieve insulin independence.
Thus, secretion signal peptide linked exendin-4 gene was constructed for gene modification of islets.[2] The viability and morphology of SP-Ex-4 transduced islets weren’t significantly different from untransduced islets. Also, the amount of insulin secreted from SP-Ex-4 transduced
islets was significantly higher than untransduced islets at high glucose stimulation (28 mM). Balb/c nude mice were chemically rendered diabetic and different numbers of SP-Ex-4 transduced and untransduced islets were transplanted under the kidney capsules. The median survival time (MST) of mice transplanted with 50 untransduced islet transplanted islets was 12 ± 3.75 days. However, the MST of SP-Ex-4 transduced islet recipients was >30 days. In conclusion, SP-Ex-4 transduced islets using lentiviral vectors can be used to improve the problem associated to the islet donor shortage in islet transplantation.