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영어

Crystallization is a simple, energy-efficient and environmentally friendly process for purifying vancomycin from fermentation broth. However, the crystallization process has been inherently problematic due to the lengthy crystallization time that is required. An improved crystallization
process could significantly reduce the crystallization time by increasing the surface area available for crystallization. Vancomycin yield was improved and the crystallization time was shortened compared to the control by increasing the surface area per working volume (S/V) of the reacting solution through the addition of a cation exchange resin (Amberlite 200), an anion exchange resin (Amberlite IRA-400), or glass beads. Most of the vancomycin could be obtained after about 12 hr of crystallization using Amberlite 200 and Amberlite IRA-400. Since high purity vancomycin can be obtained in high yield and the crystallization time can be reduced, this improved method is expected to significantly enhance the final purification process.