원문정보
초록
영어
Bombyx mori paralytic peptide (BmPP) is a multifunctional insect cytokine. BmPP consists of 23 amino acid residues including a core region that shows a structural similarity to the C-terminal β-loop domain of human epidermal growth factor (hEGF). Even though BmPP lacks the N-terminal subdomain of hEGF which is indispensable for binding EGF receptor (EGFR), BmPP showed human cell growthpromoting activity like hEGF. On the basis of this finding, a novel EGF-like growth factor was constructed by combining the N-terminal disordered region of BmPP and the C-terminal β-loop domain of hEGF. The constructed chimeric peptide consisting of 27 amino acid residues was designated "PEChimera". PEChimera increased the phosphorylated level of mitogen-activated protein kinase (MAPK) and also possessed human cell growth-promoting activity with a potency equivalent to those of hEGF and BmPP.