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영어

Nattokinase is a potent fibrinolytic enzyme that is considered to be a promising agent for thrombosis therapy. The enzymes were discovered from various sources, such as Japanese natto, Korean Chungkookjang soy sauce, and Chinese douchi. Based on its food origin and relatively strong fibrinolytic activity, nattokinase has advantages over other commercially used medicine in preventative and prolonged effects, convenient oral administration, and stability in the gastrointestinal tract. In this study, Bacillus subtilis was cultivated to produce nattokinase.
Glucose and NH4Cl were used as carbon and nitrogen sources with peptone supplementation. In flask culture, peptone concentration was fixed at 50 g/L. The highest cell growth (optical density monitored at 600 nm) and nattokinase activity were obtained with 10 g/L of glucose supplementation. At this condition, nattokinase activity was 688 unit/ml. In fed-batch culture of B. subtilis in fermentor, the ratio of peptone and glucose in feeding solution was varied. The highest cell concentration and nattokinase activity were 75 g/L and 15,000 unit/ml, respectively.
Nattokinase activity increased with cell growth, showing growthassociated production.