earticle

영어

WSSV is one of the most serious pathogens affecting the shrimp aquaculture industry worldwide. At present, at least 40 WSSV structural proteins ranging from 68 to 6077 amino acid residues in size have been identified. Viral envelop proteins of WSSV play important roles on the virus infection. This study was carried out for the identification of mRNA expression level at WSSV infection stages through comparison with various structural proteins. mRNAs of shrimps were isolated from shrimp organs at various infection stages after WSSV infection. Through the comparison of mRNA transcriptional levels of infected shrimp organs, it was confirmed that the vaccination of shrimp delayed viral gene transcription for 5 to 10 days. However, the general mRNA transcriptional pattern of vaccinated shrimps showed similar trend to that of unvaccinated
shrimps. This results indicated that VP28 was not suitable for the analysis of infection stage due to early and high level transcription in every sample. But, VP26 was a useful marker of infection stage owing to infection stage related RT-PCR transcriptional pattern. RT-PCR of VP26 could provide information on the shrimp mortality and virus propagation.