earticle

영어

The diagnosis of infectious diseases is nowadays mostly performed by molecular methods that require multiplex detection and quantification of a wide range of pathogenic microorganisms. However, simultaneous multiplex quantitative detection of pathogens suffers from compromises
between the level of multiplexing and accuracy of quantification. Here, we demonstrate the novel multiplex pathogen detection method using capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) coupled with multiplex ligation-dependent probe amplification (MLPA). MLPA-CE-SSCP is composed of four major steps which are probe hybridization, probe ligation, multiplex amplification and detection. Using ten foodborne pathogens as a model set, all the steps were carefully optimized to precisely quantify by MLPA-CE-SSCP. We could obtain the results which illustrate a strong potential in clinical diagnosis, food safety, and biosafety.