원문정보
초록
영어
Saccharification of Laminaria japonica (sea tangle) is an essential process for the use as a substrate for bioethanol fermentation. The seaweeds obtained from local market in Busan (South Korea) were dried in sunlight or hot-air and then ground by hammer mill to the powder with
150~750 ㎛ (wet) sizes of particles. The saccharification process by the thermal acid hydrolysis and the isolated marine bacteria treatment showed the highest yield of saccharification from Laminaria japonica. The optimal saccharification condition with the highest reducing sugar
content and lowest viscosity was determined as follows: 10% (w/v) seaweed slurry, 0.2% (v/v) H2SO4, thermal hydrolysis at 121℃ for 60 min, neutralization by 5N NaOH, and then incubation at 30℃, 200 rpm for 5 days after 10% (v/v) inoculation of the isolated marine bacteria to the seaweed slurry. The reducing sugar content and viscosity under the optimal condition were 40.5 g / liter and 24.9 cp, respectively. Isolated marine bacteria was identified as Bacillus sp. by 16s ribosomalRNA sequencing. The saccharification yield of Laminaria japonica was 82.1% with the optimal saccharification conditions.