원문정보
초록
영어
Single nucleotide polymorphisms (SNPs) are one-base DNA sequence variations that are responsible for various inherited diseases. DNA ligase-based mismatch detection methods have been proposed as useful strategies for SNP analyses. However, there is a critical problem for
cytosine/thymine(C/T) SNP analyses: guanine:thymine (G:T) mismatch is not distinguished from guanine:cytosine (G:C). In this study, we employed chemically modified nucleobases at the end of a ligation fragment and analyzed whether it shows differnet ligation efficiency between G:C and G:T. Successful ligation for G:C and no ligation for G:T were observed when oxanine was employed adjacent to guanine in the ligation junction. This ligation method using an oxanine-containing fragment has strong potentials for the accurate analysis of C/T SNPs.