earticle

영어

AD is the most common neurodegenerative disease. The pathological hallmark of extra-cellular β-amyloid (Aβ)deposit is considered as one of the primary factors in inducing AD. However, the mechanism of Aβ deposition on the cell membrane are the induced cytotoxicity is still unclear. In our studies, we proposed a “recruiting hypothesis” to describe the binding mechanism of Aβ or Aβ aggregates with cell membrane and the induced cell toxicity from molecular level. This presentation presents basically the isothermal titration calorimetry (ITC) studies on the Aβ aggregations, Aβ binding with liposomes with various compositions to mimic the binding with cell, and Aβ binding with PC12 cell. All these thermodynamics information obtained were discussed with the measurements of circular dichroism, surface plasmon resonance, monolayer (trough), flowcytometry, and cell viability measurements. In summary, the recruiting hypothesis is verified by this investigation and can be adapted as a molecular level understanding of the mechanism of AD and the basis of treatment developments of AD, such as drug design.