earticle

영어

Significant improvements have been reported in development of biomaterials to engineer stem cell therapeutics. Despite these advances in biomaterial development for stem cell engineering, the diversity of functional materials still remains limited, in part due to their slow, multistep
syntheses, and complicated modifications. The customization of each synthetic reaction and multiple steps limits the ability to generate a significant library size with diversity. To overcome these limitations, we have established combinatorial biomaterial libraries with chemical
approaches which allow the simple, rapid, and parallel generation of materials and screened biomaterials optimal for stem cell engineering. Compared with commercially available reagents, some of identified materials have shown higher efficiency of delivering DNA, mRNA or
siRNA for genetic modification of stem cells. These genetically engineered stem cells have exhibited enhanced therapeutic efficacy of treating cardiovascular or bone diseases. Interestingly, some of screened biomaterials could support clonal growth or specific differentiation of pluripotent stem cells. The high-throughput screening technology reported here
could provide efficient tools for developing stem cell therapeutics for various types of destructive diseases.