earticle

영어

Nanobiocatalysts, enzymes incorporated with nanostructured materials, have great potential to provide robust biocatalysts with stable and high enzymatic activity for many practical applications. The use of nanostructured materials including nanofibers, carbon nanotubes, and nanoparticles is an intriguing approach for developing nanobiocatalysts since these materials manifest great efficiency in the manipulation of the nanoscale of enzymes and thus provide a large surface area, which can lead to high volumetric enzyme activity and unprecedented stability beyond classical enzyme immobilization methods such as simple adsorption, covalently attachment or entrapment. In order to realize nanobiocatalysts the new immobilization strategy between enzymes and nanostructured materials should be considered. For these
concerns, enzyme coating approach was introduced, which is accomplished by cross-linked enzymes via glutaraldehyde mediated coupling with nanostructured materials. Recent results about the improved performance of nanobiocatalysts using various enzymes and nanostructured materials such as nanofibers, nanoporous media, carbon nanotubes, and nanoparticles will be introduced. In addition, the practical applications of nanobiocatalysts in proteomic analysis,
biosensors, biofuel cells, and bioconversion will be introduced to show the possible extension of nanobiocatalysts in various practical applications depending on the selection of appropriate enzymes and nanostructured materials. The deep understanding between enzymes and nanostructured materials will help the development of innovative nanobioctalysis approaches, which might guide to numerous practical applications of enzyme technology.