earticle

영어

The freshwater green alga Haematococcus pluvialis produces significant amounts of the ketocarotenoid astaxanthin (3,3’-dihydroxy-β,β-carotene-4,4’-dione) during the induction of green vegetative cells to red cyst cells. Compared to β-carotene, astaxanthin has two additional oxygenated groups on each ring structure and has a high antioxidant capability, reaching an
activity ten times greater than that of other carotenoids, such as β-carotene and lutein. In this study, we focused on the use of a high cell density culture of H. pluvialis based on fed-batch cultivation using an exponential feeding strategy, which is a simple but powerful method that allows cells to grow at a constant specific growth rate. From the batch cultures in a CO2-fed flat
type photobioreactor under unsynchronized illumination, the parameters for the feeding equations of the growth-limiting nutrients, nitrate and phosphate, were determined for the fed-batch cultivation, such as the cell growth yields on the nutrients and the specific cell growth rate. Under exponential feedings of the growth-limiting nutrients according to the feeding equations, H.
pluvialis was photoautotrophically cultivated in the CO2-fed flat type photobioreactor, followed by photoautotrophic induction for astaxanthin production.