원문정보
초록
영어
Crude polysaccharide (WG-PL-CP) was fractionated from fermented ginseng with Phellinus linteus in solid culture to enhance the immunostimulation of ginseng. WG-PL-CP produce three active polysaccharide-rich fractions (WGPL- CP-II, III, and IV) on DEAE-Sepharose CL-6B (Cl− form). WG-PL-CP-III displayed higher mitogenic activity (1.98- fold of the saline control at 100 μg/mL) than did WG-CP-III or PL-CP-III (1.60- or 1.65-fold, respectively), and potent intestinal immune system modulating activity through Peyer's patch was obtained by WG-PL-CP-IV only (1.56-fold). Meanwhile, WG-PL-CP-II and III significantly enhanced macrophage stimulating activity (2.01- and 1.94-fold) compared to WG-CP-II and III (1.73- and 1.66-fold) or PL-CP-II and III (1.79- and 1.72-fold). In addition, WG-PL-CP-III and IV mainly contained neutral sugar (73.5 and 67.3%) and uronic acid (23.2 and 24.6%). Component sugar analysis also showed that WG-PL-CP-III consisted mainly of uronic acid as well as the neutral sugars Glc, Ara, Gal, Rha and Xyl (molar ratio of 0.81:1.00:0.49:0.42:0.28:0.20), whereas WG-PL-CP-IV was mainly comprised of uronic acid, Ara, Rha, Gal, Xyl and Glc (1.00:0.75:0.69:0.63:0.42:0.34). Therefore, it is assumed that these active polysaccharides play an important role in enhancing the immunostimulation of fermented ginseng with P. linteus in solid culture.
목차
서론
재료 및 방법
수삼의 상황버섯 균사체 고체배양을 통한 발효물 조제
수삼-상황버섯 균사체 발효물로부터 열수추출물과 조다당획분조제
수삼-상황버섯 균사체 발효물 조다당으로부터 면역활성 다당획분의 분획
면역활성 다당획분의 성분 및 구성당 분석
실험동물과 시약
비장세포의 마이토젠 활성
Peyer’s patch를 경유한 장관면역 활성
마크로파지 활성
통계처리
결과 및 고찰
수삼-상황버섯 균사체 발효물의 열수추출물 및 다당체의 면역활성
수삼-상황버섯 균사체 발효물로부터 얻은 다당체 분획에 따른면역활성
수삼-상황버섯 균사체 발효물로부터 얻은 면역활성 다당체 획분의 특성
요약
문헌