원문정보
초록
영어
Picrate, enzyme-picrate and instrumental analysis methods using IC (Ion Chromatography) and HPLC (High Performance Liquid Chromatography) were compared for their effectiveness in determining cyanide in extracts of Maesil, which is classified as a harmful substance. First, the picrate method showed the shortest analysis time (about 5 hr). The color of picrate paper changed at 0.01 mg/200 mL CN−. However, it was difficult to detect cyanide from amygdalin of glucosides. Second, we performed a qualitative analysis for total cyanide (free cyanide and cyanide from amygdalin) by the enzyme-picrate method using β-glucosidase and a quantitative analysis by spectrophotometry. Finally, analysis of cyanide by IC and HPLC required the longest determining time (about 17 hr) as well as pretreatment for each free cyanide and amygdalin. These results suggest that enzyme-picrate is the most effective analysis method for the detection of cyanide in Maesil extracts.
목차
서론
재료 및 방법
실험재료
시약 및 표준물질
피크린산지(紙)법
효소-피크린산지법
아미그달린의 분리 및 HPLC 분석
시안화합물의 분리 및 IC/HPLC 분석
분석법의 유효성 검증
결과 및 고찰
피크린산지법
효소-피크린산지법
크로마토그램을 이용한 시안화합물 분석
성분의 확인
시험법 비교
요약
문헌