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The development of the alternative of fossil fuel is a hot issue. Especially, bio-ethanol is produced using food crops as a renewable resource and bio-ethanol is already commercialized for fuel in South America and Europe. However, bio – ethanol production from biomass needs a complicate and expensive pretreatment procedure for saccharification of lignin, cellulose, hemicelluloses. In the view point of food resource, it causes molar problem. Therefore we tried to produce bio–ethanol using the waste from beer culture broth without the pre treatment procedure. In previous study, it took much time to produce bio-ethanol using the waste from beer culture broth at moderate temperature because the saccharification rate is too low. In this study the simultaneous saccharification and fermentation was carried at high temperature in order to improve the saccharification rate because the optimum temperature of saccharification using α,β-amylase(starch hydrolysis) was at least higher than 60 oC. As a result, fermentation time
was shorter. The production of byproducts, acetic acid and acetaldehyde was also investigated.