원문정보
초록
영어
As nucleic acids alone can’t penetrate the cell membrane, efficient carriers are needed. Therefore, we synthesized a probe for oligonucleotides and imaging using aminated APTS coated silica nanoparticles, modified with FITC-doped and cell-penetrating peptides (HIV-1 trans-activator factor (TAT)). In addition, the dye-functionalized SiNPs could make easily control of various sizes of 10nm~800nm. Here, we prepared TATfunctionalized SiNPs conjugated Cy5 labeled DNA fragments and TAT peptide for the purpose of improving intracellular delivery. By showing that SiNP-DNA probes were internalized by cells and distributed intracellularly thoughout the cytoplasm and not penetrate the nucleus. This study demonstrates the feasibility of using SiNPs as a potential carrier for therapeutic gene delivery.