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화장품

Development of SPRi-Protein Chip for Depigmenting Agent Screening

원문정보

Seunghak BAEK, Man-ki SON, Babitha SUMATHY, Hyang-Bok LEE, Dung Hoang NGUYEN, Yu ri LEE, Eun-Ki KIM

한국생물공학회 한국생물공학회 학술대회 2009 추계학술대회 및 국제심포지움 2009.11 pp.247-247
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

Microphthalmia-associated transcription factor (MITF), a basic Helix-Loop Helix (HLH) transcription factor with a leucine zipper, is a key regulatory protein for the pigmenting process in melanocytes. Binding of MITF to E-box caused transcription of several pigmenting genes
including the tyrosinase gene. The purpose of this study was to analyze the interaction of MITF and its DNA binding site (M-box) by using surface plasmon resonance (SPR) imaging. We developed DNAprotein interaction arrays, coated with polypeptide poly(L-lysine) with a bifunctional coupling property that permits efficient immobilization of 1,4-phenylenediisothiocyanate on gold surfaces. DNA arrays on modified gold surfaces were fabricated, and then used for the analysis of DNA-MITF interaction. It is expected that SPR imaging can be used as a powerful tool to study DNA-protein interactions.

키워드

저자정보

  • Seunghak BAEK Dept. of Biotechnology, Inha University, Incheon.
  • Man-ki SON Dept. of Biotechnology, Inha University, Incheon.
  • Babitha SUMATHY Dept. of Biotechnology, Inha University, Incheon.
  • Hyang-Bok LEE Dept. of Biotechnology, Inha University, Incheon.
  • Dung Hoang NGUYEN Dept. of Biotechnology, Inha University, Incheon.
  • Yu ri LEE Dept. of Biotechnology, Inha University, Incheon.
  • Eun-Ki KIM Dept. of Biotechnology, Inha University, Incheon.

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자료제공 : 네이버학술정보

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