earticle

영어

Chronic exposure of Ultraviolet (UV) leads to a variety of skin damage such as sun-burn, pigmentation, premature aging and photocarcinogenesis. Moreover, the response of inflammation and photo-damage against UV induced skin can be aggravated by reactive oxygen species (ROS) induced by UV irradiation. Therefore, the importance of the protection effect by UV damage has been regarded continuously as well as the development of effective material that can mitigate the skin damage has been required. Ficus deltoidea, known as “Mas cotec”, has been widely used as several medications in Malaysia. Also, several studies have demonstrated that F. deltoidea has strong anti-oxidant activity. In this respect, our study focused on the photo-protective efficacy; particularly, we evaluated the antiinflammation effect of F. deltoidea extract on the UVB-induced skin cell. To investigate the anti-inflammatory effect, we examined the production of the inflammatory cytokines such as such as tumor necrotic factor-α (TNF-α),
interleukin-1α (IL-1α), IL-6 by enzyme-linked immunosorbent assay (ELISA). Also, we examined the expression of COX-2 after UVB irradiation by western blot assay. We found that the amount of these inflammatory cytokines in the HaCaT keratinocytes was significantly reduced by treatment of F. deltoidea extract after UVB irradiation. Along with this result, treatment with F. deltoidea extract inhibited COX-2 expression by UVB irradiation in a dose dependent
manner. These results point to the potential use of F. deltoidea extract as agent for cutaneous inflammation response