earticle

영어

Parylene H layer was deposited on surface of protein chip to promote its immobilization efficiency. To construct immunoaffinity layer, protein chip made from glass substrate requires surface modifications such as silanization or depositing polymer film on substrates which
have been developed1). Through preparing parylene H layer on glass substrate, aldehyde (-CHO) functional groups are exposed on surface of the protein chip. Aldehyde fuctional groups are make covalent bonding with most kinds of proteins and peptides which contain amine
groups. Its effectiveness was determined by quantification of immobilized horseradish peroxidase (HRP) compared with polystyrene. For medical diagnostic experiment we detect antibodies against cyclic-citrullinated peptide (CCP) in sera from patients of rheumatoid arthritis (RA) on protein chip by using microarrayer and scanner. The results are compared with those of ELISA assay.