earticle

영어

Cell-based assays are increasingly being used in drug discovery, genetic analysis and single-cell analysis. Cell microarrays can be classified into two general categories: chip-based planar arrays and particle-based suspension arrays. Although chip-based planar arrays have been widely used, suspension arrays are becoming popular as an alternative approach due to several advantages over planar array. In this study, we prepared suspension microarrays of cells using various shape of SU-8 microparticles and demonstrate the potential use of this system for multiplexed cell-based assay. Different size and shape of SU-8 50 negative photoresist was micropatterned on the glass slide using typical photolithography. SU-8 microparticles were obtained by detaching micropattern by ultrasonication. Resultant microparticles were incubated with different cell types. Cells adhered on SU-8 microparticles were observed with optical microscopy and a live/dead viability/cytotoxicity fluorescence assay. In this study, three different
cell types were immobilized on the three different shapes of microparticles so that each cell type was easily identified. Capability of controlling the shapes of microparticles opened the possibility of producing shape-coded suspension array, which can offer a costeffective and versatile platform for high-throughput and multiplexed cell-based assay.