원문정보
초록
영어
In this work a microplate-based bioreactor equipped with the optical online monitoring system(MABOOMS) has been investigated for high-throughput screening of microorganisms and medium optimization in biotecnological processes for online monitoring of optical density
(OD). An optical density sensing system has been made with 630 nm LED(light-emitting diode) and PMT(photomultiplier tube). It has been used to detect the reflect light intensity of a culture medium in 24-well microplate. A 24-well microplate has been employed to online monitor
the optical density of fermentation processes with E.coli DH5α and B.cereus 318. The online monitoring data of the optical density have been compared with offline measurement data by using spectrophotometer. The tendency of the online data agreed with offline data, that is, the
24-well microplate based bioreactor was validated to be very useful to online monitor the cell growth characteristics.