원문정보
초록
영어
Increasing interest is now set in clinical applications of human mesenchymal stem cells (MSC). In order to explore there usages in medical applications, the ex vivo expansion of MSCs to sufficient cell numbers is necessary. Microcarrier cultures have been shown to allow
extensive cell expansion of tissue engineering relevant cells, such as chondrocytes, while maintaining their phenotype. Our aim was to investigate the in vitro three-dimensional expansion of cord blooddrived mesenchymal stem cells using Hellex microcarrier. Hillex microcarrier showed the results for adherence with over 80% adherent cells after 3 h of incubation. We also cultivated the MSCs in 12 well plate to comparison with two dimensional monolayer cultivation.
Results shown that the three dimensional expansion of MSC on microcarriers represents a beneficial alternative to the conventional two dimensional monolayer cultivation method.References 1. M. Du¨ rrschmid, K. Landauer, G. Simic, G. Blu¨ml and O.
Doblhoff Dier Strategies for Microcarrier Based Animal Cell Bioprocesses BIOTECHNOLOGY AND BIOENGINEERING, VOL. 83, NO. 6, SEPTEMBER 20, 2003 681-686S. Tielens, H. Declercq, T.
Gorski, E. Lippens, E. Schacht and M. Cornelissen Gelatin-Based Microcarriers as Embryonic Stem Cell Delivery System in Bone Tissue Engineering: An in-Vitro Study BIOMACROMOLECULES 2007, 8, 825-832.