earticle

영어

Mucosal vaccination has been considered as an attractive immunization principle based on the consideration that mucosal surfaces are vulnerable for invasion by pathogen. In that sense, transgenic plant is noticed as promising carrier offering attractive option for production and delivery of mucosal vaccine. Classical swine fever (CSF) is a highly contagious viral
disease of pigs and results in devastating financial losses. Because CSFV E2 glycoprotein mediates the viral attachment and penetration to susceptible cells, it is considered as a target for inducing protective immunity. In this study, in order to develop the vaccine materials for
mucosal immunization against the CSFV infection, rice calli expressing E2 were established. Among them, p15B, not expressing KDEL, and p16B, expressing KDEL, express E2 protein of about 5 mg/mg dry weight. In addition, the proteins expressed in the transgenicrice were immunogenic in mice. More importantly, recombinant E2 conjugated with M cell ligand
targeting ligand, Co1, effectively induced immune response after oral immunization of the mice. Currently, we are analyzing immune responses induced by oral administration of E2-expressing rice cells. (This work was supported by the grant from ARPC.)