원문정보
초록
영어
Abnormal glycosylations are closely associated with human cancers. And certain types of glycan structures are well-known markers for tumor progression. Therefore, the highly sensitive and high-throughput analysis of glycans from human body fluids is a growing issue in glycomics. In this study, we present a rapid and high-throughput human serum N-glycan release and purification technology using 96-well plate based procedures. The N-glycans released from a polyvinylidene fluoride membrane filter plate were subsequently loaded onto
porous graphitic carbon containing 96-well plate to remove the salts and other contaminants. The purified N-glycans were characterized by qualitative and quantitative analysis using matrix-assisted laser desorption/ionization time-offlight mass spectrometry and carboxymethyl trimethylammonium hydrazide derivatization that was introduced to generate a permanent cationic charge to the reducing end of neutral oligosaccharides. Finally, this robust N-glycan
preparation technology was applied to ovarian cancer diagnosis using 5 ul of patient serum. It is believed that the established 96-well plate platform described here may contribute to the rapid screening of potential N-glycan biomarkers in a large number of human patient serum sample sets.