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논문검색

동물 및 식물세포공학

Improvement of Recombinant trypsin Production by Suppressing of Expression of Cysteine Proteinase Gene using RNA interference

초록

영어

Recombinant protein production in plant cell suspension culture is limited by secreted proteases with high level. To overcome this problem, we have been previously identified secreted protease and reported that the cysteine proteinase (CysP) was one of the main secreted protease
after sugar starvation in the rice suspension culture. It was investigated whether the production of trypsin in rice cell line (pMYT111-04) which stably produced trypsin could be improved by suppressing expression of CysP gene. The suspension culture of rice cell transformed with both the trypsin and a dsRNA of CysP contained reduced total protease and CysP activities, and increased trypsin production levels compared those of cell lines harboring the trypsin
only. The production of trypsin increased by up to 2 folds at 5 days using RNAi technology Acknowledgements This work was supported by a grant from the Next Generation New Technology Development program of the Ministry of Commerce, Industry and Energy.

저자정보

  • Hwa-Young YU Division of Biological Sciences, Chonbuk National University
  • Nan-Sun KIM Division of Biological Sciences, Chonbuk National University
  • Moon-Sik YANG Division of Biological Sciences, Chonbuk National University

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