원문정보
초록
영어
Recombinant protein production in plant cell suspension culture is limited by secreted proteases with high level. To overcome this problem, we have been previously identified secreted protease and reported that the cysteine proteinase (CysP) was one of the main secreted protease
after sugar starvation in the rice suspension culture. It was investigated whether the production of trypsin in rice cell line (pMYT111-04) which stably produced trypsin could be improved by suppressing expression of CysP gene. The suspension culture of rice cell transformed with both the trypsin and a dsRNA of CysP contained reduced total protease and CysP activities, and increased trypsin production levels compared those of cell lines harboring the trypsin
only. The production of trypsin increased by up to 2 folds at 5 days using RNAi technology Acknowledgements This work was supported by a grant from the Next Generation New Technology Development program of the Ministry of Commerce, Industry and Energy.