원문정보
초록
영어
Plastic cell culture systems have a useful tool in research and biotechnlogy process, and a novel pneumatic bioteactor system (PBS) has been recently developed by PBS Biotech, Inc. In this study, to investigate the effects of agation speeds in 2L scale PBS, various rpm
settings (10, 20 and 30) were used for cultures of rCHO cells producing monoclonal antibody (MAb). The maximum cell density in PBS at 20 rpm reached 3.4 X 106 cells/ml, and final MAb concentration was 141 mg/L. Also, when culture of commercially available suspension cell line CHO-S (Invitrogen) was evaluated in PBS, the maximum cell density reached 10.5 X 106 cells/ml. similar result was obtained in stirred tank reactor (STR, 9.6 X 106 cells/ml), which was better than in Erlenmeyer flask (5.4 X 106 cells/ml). These results revealed that the novel PBS is a strong candidate with variety of advantages such as low cost and easy scale-up up to a few thousands liters in cell cultures compated to comventional STR. Reference. 1. Sing V. (1999) Disposable bioreactor for cell culture using wave-induced agitation. Cytotechnoloty. 30:149-158