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논문검색

동물 및 식물세포공학

N-linked glycan analysis of human granulocyte-macrophage colony-stimulating factor (hGM-CSF) secreted from rice cell suspension culture

원문정보

Yun-Ji SHIN, Yun-Jo CHUNG, Kyu-Boem HAN, Kang-Yeol YU, Moon-Sik YANG, Tae-Ho KWON

한국생물공학회 한국생물공학회 학술대회 2009 추계학술대회 및 국제심포지움 2009.11 pp.228-228
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초록

영어

The N-glycans of human granulocyte-macrophage colony-stimulating factor produced in genetically engineered rice cell suspension culture were analyzed. In previous study, immunoblot assay detection of secreted hGM-CSF revealed the presence of multiple glycoforms with molecular weights higher than observed for E.coli-derived hGM-CSF. In this study, N-glycan patterns of total secreted proteins were analyzed and then N-glycan patterns of secreted hGM-CSF were examined using HPLC and MALDI-TOF mass spectrometry. From the
rice-derived hGM-CSF, seven types of N-glycns were MMXF3 (30.96%), GnMXF3 (44.76%), GnMX (1.84%), GnGnXF3 (8.73%), AF4MXF3 (4.76%), AGnXF3 (7.20%) and AF4GnXF3 (1.75%). The ratios of paucimannosidic-type (MMXF3) and complex-type glycoforms were 30%, 70%, respectively and nearly 20% of complex N-glycans showed Lewis a structure (This work was supported by the grant from Korean Ministry of Knowledge Economy)..

키워드

저자정보

  • Yun-Ji SHIN Jeonju Biomaterials Institute, Jeonju 561-360, KOREA.
  • Yun-Jo CHUNG Center for University-wide Research Facilities, Chonbuk National University, Jeonju 561-756, KOREA.
  • Kyu-Boem HAN Hanson Biotech Co., Ltd.
  • Kang-Yeol YU Jeonju Biomaterials Institute, Jeonju 561-360, KOREA.
  • Moon-Sik YANG Division of Biological Sciences, Chonbuk National University, Jeonju 561-756, KOREA.
  • Tae-Ho KWON Center for University-wide Research Facilities, Chonbuk National University

참고문헌

자료제공 : 네이버학술정보

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