earticle

영어

A rice cell suspension culture system with the RAmy3D promoter, which is induced by sucrose starvation, has been previously utilized to produce large quantities of recombinant proteins. However, using rice as production system for therapeutic proteins requires modification of
their N-glycosylation pattern because of the immunogenicity of plantspecific sugar residues. In this study, for generating glyco-engineered rice as production host for therapeutic glycoproteins, an intron-containing self-complementary hairpin RNA-mediated post transcriptional gene
silencing was used to obtain a targeted down-regulation of the endogenous glycosyltransferase genes in rice cell suspension cultures. N-linked glycans from the generated RNAi lines were identified and their structures were compared with those isolated from the wild type
suspension cells. The Δ3FT/XT glyco-engineered line with significantly reduced xylosylated and/or core α1,3-fucosylated glycan structures. In case of the Δ4FT/GT glyco-engineered cell lines, showed that completely suppressed of Lewis a epitopes (This work was supported
by the grant from JBMI).