earticle

영어

The pathogenicity of the insect-parasitic nematode Butlerius sp. ANU501 was evaluated against three major cruciferous insect pests: Artogeia rapae (Lepidoptera: Pieridae), Mamestra brassicae (Lepidoptera: Noctuidae), and Plutella xylostella (Lepidoptera: Yponomeutidae). All
insect species were highly susceptible to Butlerius sp. ANU501 and the larval mortality increased with an increasing dosage of nematodes under petri dish experiment. The pathogenic effect of nematodes was higher for the 4th instars than those for the corresponding 3rd instars in the order of P. xylostella, A. rapae, and M. brassicae, with the highest mortalities of 93.5, 88.2, and 77.8%, respectively. LD50 values of Butlerius sp. ANU501 against 4th instars of P. xylostella, A. rapae,
and M. brassicae were 33.1, 36.1, and 48.2, and LT50 of 33.6, 41.1, and 61.5 h, respectively. The nematodes established in host varied significantly with insect species in P. xylostella, A. rapae, and M. brassicae (e.g., 6.9, 4.1, and 3.7 at a dose of 40 nematodes per larva for 3rd instars and 8.9, 5.7, and 4.6 at 80 nematodes per larva for 4th instars). Overall, Butlerius sp. ANU501 caused higher pathogenicity in P. xylostella, than in the other insect species.