원문정보
초록
영어
In general, Industrial yeast strains used for baking or brewing lack selectable genetic marker and identification of hybrids by genetic complementation is impossible. Selection markers can be introduced by mutation into industrial strains. In the present study, respiratory deficient mutant and auxotrophic mutant for selection maker were induced by acriflavine treatment and mutagenesis with NTG, EMS, repectively. As a result, respiratory deficient petite mutants of industrial ethanol fermentative yeast, Saccharomyces cerevisiae CHY1011, CHFY0321 and Auxotroph of thermostable yeast, Kluyveromyces marxianus KCTC17631 have been isolated. The yeast strains can be used for protoplast fusion between S. cerevisiae CHY1011RD,
CHFY0321RD and K. marxianus CHY1703 using tetrazolium overlay technique and selection medium.