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Terminal-Restriction Fragment Length Polymorphisms (T-RFLP) Analysis of Microbial Community in Methane Gas Production Process

초록

영어

Anaerobic digesters for the methane gas production from food wastes were comprised with methane fermentation, stabilized reactor. During one years, 25 Samples were collected from the reactors and the microbial communities were analyzed by T-RFLP using two different
primer sets (63F-FAM/1389R and 27F-FAM/1492R) and three restriction enzymes (AluI, RsaI, and HaeI). Among these combinations, the combination of 63F-FAM/1389R primer set and restriction enzyme AluI showed the highest discrimination level of the microbial community and T-RFLP analyses with this combination could be used as a method for addressing microbial diversity of the anaerobic digesters.

저자정보

  • Bae JAE-SANG Center for Environmental Technology Research, Korea Institute of Science and Technology.
  • Kim SE-IL Center for Environmental Technology Research, Korea Institute of Science and Technology.
  • Ju DONG-HUN Environmental R&D Center, Hansol EME.
  • Seong HYEON-JE Environmental R&D Center, Hansol EME.
  • Park SEONG-BEOM Environmental R&D Center, Hansol EME.
  • Um YOUNG-SOON Center for Environmental Technology Research, Korea Institute of Science and Technology.
  • Sang BYOUNG-IN Center for Environmental Technology Research, Korea Institute of Science and Technology.

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