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The purpose of this study was to investigate the possibility of enhancing biological activities of fresh ginseng which has no commercial value. Fresh ginseng was extracted under high pressure extraction condition at 80℃ and 120 bar for 20min. On the basis of HPLC analysis, amounts of ginsenosides in fresh ginseng were increased up to 10-30% by High Pressure Extraction (HPE) process, compared to conventional water extraction at 100℃. Quantitatively, amounts of Rh1 and Rg3 which were main protopanaxatriol ginsenosides, were measured as 620.08ppm and 313.97ppm. Rh2, Rg2 and Rf which were protopanaxadiol ginsenosdies group were also increased up to 665.16ppm, 415.21ppm, and 477.34ppm, respectively. The cytotoxicity of the extracts on human normal kidney cell, HEK293 was 11.3% which was much lower than those from conventional extraction processes in adding 1.0 ㎎/㎖ of the highest concentration. Human stomach adenocarcinoma and human lung carcinoma cell growth were inhibited up
to 80% and 87%, respectively in adding 1.0 mg/ml of extracts. These values were higher 30-40% than those from water extraction at 100℃. It was found that fresh ginseng associated with high pressure extraction process could improve its anticancer activities because the extraction process could generate more active compounds of Rg1, Rg2, Rh1, Rh2, Re, Rf and Rd than those from normal extractions. Some compounds such as Rg1, Rg2, Rh1 and Rh2 were found in the extracts from high pressure process in fresh ginseng