원문정보
초록
영어
Immuno-modulatory activities of fresh ginseng could be improved by employing ultrasonification extraction process. Fresh ginseng was extracted at 60℃ with water for 60min, and then treated with ultrasonification at 40 kHz for 15min. From HPLC analysis, amounts of ginsenosides, Rb1, Rb2, Rd, Rg1, Re, and Rf were increased up to 2-3 times, compared to these from
conventional water extraction. The extracts also showed low cytotoxicity on human kidney cell (HEK293) as 15%. For human B and T cell growth, fresh ginseng extracted by ultrasonification process showed the highest cell growth as 9.33ⅹ104 cells/ml and 8.50ⅹ104 cells/ml, respectively, which were increased up to 35% compared to those from conventional extraction process. The secretion of cytokine for IL-6 and TNF-αfrom human T cell were also increased to 8.5ⅹ10-4 pg/ml and 8.3ⅹ10-4 pg/ml, respectively in adding the extracts. Based HPLC peaks comparison between crude extracts and the extracts treated with ultrasonification, ultrasonification process definitely plays an important role in effectively extracting biologically active compounds, which could result in improving immuno-modulatory activities.