원문정보
초록
영어
This study was to improve immune activities of Acer mono by employing high energy extraction using 40 ㎑ ultrasonific wave at 60℃. The cytotoxicity of all extracts on normal human cell(HEL299) was lower than 20% in adding 1.0 mg/㎖. Especially, the extracts from ultrasonic extraction showed the lowest cytotoxicity as 15% in adding 1.0 mg/㎖. The growth of B cell in adding water extracts were observed as 4.1×104 cells/㎖ and ultrasonification extracts as 5.9×104 cells/㎖. Generally, the growth of human immune B and T cell in adding the ultrasonification extracts was increased up to 40%, compared to those from water extraction. The secretion of IL-6 and TNF-α from those cells were also enhanced up to 30~40% by ultrasonification extraction process. As a result of in vivo experiments, IgG levels in the blood was enhanced to 44.2 ng/㎖ in daily feeding of 100 mg/㎖ to ICR mouse, while the water extract secreted IgG level as 29.5 ng/㎖. In conclusion, ultrasonic extraction process could
improve its immune activities because of less heat damages during the process.