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영어

Basic characteristics of astaxanthin and Haematococcus pluvialis cracked cell were investigated, and the extraction conditions were optimized for the astaxanthin production from H. pluvialis cracked cells. First, the chemical compositions of H. pluvialis cracked cell, based on
dry matter were 55.5% of carbohydrate, 16.64% of crude lipid and 13.41% of crude protein. Sugar composition of H. pluvialis cracked cell was glucose (62.3%), mannose (16.6%), galactose (10.5%) and fucose (5.6%). The extraction of astaxanthin from H. pluvialis was examined
using various solvents, such as acetone, ethanol, dichloromethane and methanol. Acetone was selected as the best solvent for the extraction of astaxanthin from H. pluvialis. Response surface methodology (RSM), based on a two-factor interaction model, was applied, and optimization
of the extraction parameters such as β-glucanase concentration and sonication time was performed. Astaxanthin yields was the highest when it was extracted after H. pluvialis cell was treated by β-glucanase (2%) and the sonication was carried out spontaneously with the extraction for 75 min.