earticle

영어

Acarbose fructoside (acarbose-F1) was synthesized by the acceptor reaction of a levansucrase from L. mesenteroides B-512 FMC M1FT with acarbose and sucrose. The derivative was purified by P-2 column chromatography and HPLC, and the structure was confirmed to be 1I-β-D-fructofuranosyl-α-acarbose after 1H, 13C, HSQC, HMBC analyses. The inhibitory effects of
acarbose-F1 on 9 different starch hydrolytic enzymes (20 different sources) and on a dextransucrase were kinetically characterized and compared with acarbose. Acarbose or acarbose-F1 inhibited the reactions of α-glucosidase, amyloglucosidase, α-amylase, CGTase and dextransucrase but did not affect the reactions of β-glucosidase, β-amylase, pullulanase, isoamylase, or dextranase. Compared to acarbose, acarbose-F1 exhibited stronger inhibitions
on α-glucosidases from rice or A. niger, with competitive patterns and on amyloglucosidase from A. niger, or α-amylase from A. oryzae with mixed noncompetitive patterns. In addition, acarbose-F1 was a novel substrate for dextransucrase with Km and Vmax values of 189.0 mM and 8.51 μmol/mg/min, respectively. Although acarbose-F1 inhibited dextransucrase activity in
sucrose reaction digest resulting dextransucrase activity of 18.3% compared to without acarbose-F1, the acarbose-F1 inhibition was smaller than acarbose resulting of 2.9% dextransucrase activity compared to without acarbose.