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Reactive oxygen species (ROS) have been shown to damage tissues. Thus, ROS are associated with tissue damage and the prime contributing factors for inflammation, hypertension and diabetes, aging, cancer, arteriosclerosis. Some mushrooms, including Phellinus linteus and
Inonotus hispidus, commonly produce a bundle of yellow antioxidant pigments that are composed of hispidin derivatives and polyphenols. Hispidin, metabolite of Phellinus linteus, is a potent antioxidant and inhibitor of protein kinase C (PKC). This study investigated the
cytoprotective effect of hispidin against oxidative cell damage on rat insulinoma RINm5F pancreatic cells. Cytoprotective effect of hispidin in rat insulinoma RINm5F pancreatic cells were investigated in vitro. Various methods for the radical-scavenging activities of the hispidin
were examined by using α,α-diphenyl-β-picrylhydrazyl (DPPH) radicals and 2,2'-azino-bis(3-ethylbenzthiazoline- 6-sulfonic acid) (ABTS) assay. After cell viability was measured to decide the proper sample concentration, no cytotoxicity was shown in rat insulinoma RINm5F
pancreatic cells using MTT assay and 2', 7'-Dichlorodihydrofluorescein diacetate(DCFDA) assay. And hispidin scavenged intracellular ROS and thus prevented oxidative stress-induced apoptosis.