원문정보
초록
영어
Brown alga Sargassum siliquastrum was extracted for 2 day with a mixture of acetone-dichloromethane (1:1), followed by extraction of the residue for an additional 2 days with methanol. The combined crude extracts of Sagassum siliquastrum were fractionated with nhexane, 85% aq MeOH, n-BuOH, and H2O. Further purification of 85% aq MeOH fraction led to the isolation of two isomeric chromanols, sargachromanols D and E, whose structures were determined by 2 D NMR experiments such as 1H COSY, TOCSY, gHMQC, and gHMBC. Sargachromanols D and E exhibited potent scavenging activity on the authentic ONOO- as well as ONOO- induced from SIN-1. Also, they showed good scavenging effect on DPPH radical, comparable to BHA as a positive control. Sargachromanol E showed higher scavenging acitvities on the DPPH radical and peroxynitrite than sargachromanol D.