earticle

영어

Whole plants of Corydalis heterocarpa were collected at Heunri Jeollanamdo, Korea, in July, 2003. The collected samples were dried under the shade and extracted twice with dichloromethane and methanol, in turn. The combined crude extracts were concentrated in
vacuo and then partitioned between dichloromethane and water. The organic layer was fractionated with n-hexane and 85% aq. methanol, and the aqueous fraction was also further fractionated with n-butanol and water, successively. The antioxidant activities of crude extracts
and their solvent fractions were determined by evaluating DPPH radical, authentic ONOO-, and ONOO- generated from SIN-1 (3-morpholinsydnonimine) in vitro as well as the degree of occurrence of intracellular nitric oxide (NO). Scavenging activities of solvent fractions
on authentic ONOO- increased in the order of n-butanol > 85% aq. methanol > H2O > n-hexane fractions, while those on ONOOgenerated from SIN-1 increased in the order of n-butanol > H2O >
85% aq. methanol > n-hexane fractions. In addition, all solvent fractions effectively inhibited the intracellular NO levels. These results suggested that C. heterocarpa contain compounds with strong radical scavenging and antiradical-generating effects.