원문정보
초록
영어
Calcium phosphate has been used for over 30 years to deliver genetic material to mammalian cells. The traditional method using calcium phosphate strongly depends on the experimental parameters such as concentrations (DNA, salt), temperature, pH, reaction time for precipitation and also on person-to-person variation. In this study, to evaluate the effects of particle size on transfection efficiency, factors that affect transfection efficiency were carefully evaluated. Particularly, when plasmid DNA with filtered nano-particle was transfected to HEK293 cells, it was shown that the Ca-phosphate/DNA complexes were effectively transfected to the cells. We observed transfection efficiency by filtered Ca-phosphate particle with DNA varied according
to the pore size filters (0.2 μm, 0.45 μm, 0.5 μm and 1 μm).When Caphosphate particles were filtered after preparation, particles could be stored for longer than one month without significant loss in transfection efficiency. This long-term stability is one of the important features that should be satisfied to be used as gene delivery tools for gene therapy applications