원문정보
초록
영어
Butanol, which is a primary alcohol with a C4-structure (C4H10O), as a liquid biofuel has very attractive performances including high energy density, phase stability on blending with other fuels, and noncorrosiveness. Clostridium acetobutylicum ATCC 824 is one of the major butanol producers. To improve butanol formation of C. acetobutylicum ATCC 824, a gene knockout system for metabolic engineering was constructed by using an L1.LtrB group II intron from
Lactococcus lactis. The gene knockout plasmid pCACYS3 was constructed by cloning the L1.LtrB group II intron into the pIMPH, which was generated by removing both HindIII restriction sites from C. acetobutylicum-Escherichia coli shuttle vector pIMP1. The transcription of the L1.LtrB group II intron on pCACYS3 was controlled by a thl promoter. To verify the knockout system, pCACYS3, buk gene encoding the butyrate kinase was disrupted in ATCC 824 strain. [This work was supported by the Ministry of Knowledge Economy grant funded by the
Korea government (#10030795). Further supports by the GS-Caltex and the BioFuelChem].