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Elementary Mode Analysis of a Central Metabolic Network of Cyanothece ATCC 51148 and Optimized Flux Distributions for Hydrogen Production

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From the genomic sequence of Cyanothece ATCC 51148 [1], a central metabolic pathway was re-constructed by comparing to the previously known metabolic pathways in KEGG and by BLAST searching. Glycerate-3-phosphate dehydrogenase [EC1.2.1.13] utilizing NADPH as a cofactor and sedoheptulosebisphosphatase [EC:3.1.3.37] were not found thus the carbon dioxide fixation pathway was proposed to consist of pentose phosphate pathway and glycerate-3-
phosphate dehydrogenase [EC1.2.1.12] in glycolysis. An elementary mode analysis for the metabolic network was carried out to identify the optimized flux distribution for hydrogen production. A metatool analysis generated 675 modes of pathways for chemoheterotrophic growth using glycerol and nitrate. Hydrogen production and simultaneous cell growth was stoichiometically and thermodynamically possible in 126 modes. The mode of pathway was visualized as each symbol in a plot of inverse cell yield per glucose and oxygen. At the
optimized flux distribution in the central metabolic pathway, the theoretical yields of hydrogen production were calculated to be 700 mmol H2/g cell and 5.3 mol H2/g glycerol. Glycerol flows back to the glycolytic pathway and is metabolized in TCA cycle via the oxidative PPP and RuBP carboxylase because glyceraldehyde 3-phosphate dehydrogenase is inactive in the optimized flux distribution. Hydrogen is produced from the NAD(P)H generated by pyruvate dehydrogenase, malate dehydrogenase, gycerol-3-phosphate dehydrogenase, glucose-6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase.

저자정보

  • Won HUR Dept. Bioengineering and Technology, Kangwon National University

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